Arabidopsis seeds of wild-type Col-0, wox5-1 wox7-1 and plt1-21 plt2-21 double mutant were sterilized with 75% ethanol and then germinated vertically and grown on 1/2 Murashige & Skoog basal (MS) medium without sucrose at 22 ˚C under dark conditions for 9 d. The hypocotyl explants (~1 cm in length) were cut and cultured on CIM (MS basal medium with vitamins, 2% w/v sucrose, 11 µM 2,4-dichlorophenoxyacetic acid, 0.2 µM kinetin) under 24-h light conditions at 22 ˚C for 6 d. Total RNA from CIM 0 d (d0, hypocotyls) and CIM 6 d (C6, calli) were extracted with 2 biological replicates for RNA-seq.
Arabidopsis seeds of wild-type Col-0 or the
mutant were germinated on
1/2 MS medium with 1% sucrose at 22 ˚C under a 16-h light/8-h dark photoperiod.
The first pair of rosette leaves were detached from 12-day-old seedlings and
cultured on CIM at 22°C under continuous dark for 4 d to induce callus formation.
Leaf explants of Col-0 or
cultured on CIM for 0 or 4 d were collected
Citation: Plant Cell Physiol. 59(4):739-748. GEO accession number: GSE101321